Compactly
self-wired neuronal networks
This project concerns with a novel approach for patterning
cultured neural networks in which a particular geometry is achieved via
anchoring of cell clusters (tens of cells/each) at specific positions. Compact connections
among pairs of clusters occur spontaneously through a single non-adherent
straight bundle composed of axons and dendrites. The anchors that stabilize the
cell clusters are either poly-D-lysine, a strong
adhesive substrate, or carbon nanotubes. Square, triangular and circular
structures of connectivity were successfully realized. Monitoring the dynamics
of the forming networks in real time revealed that the self-assembly process is
mainly driven by the ability of the neuronal cell clusters to move away from
each other while continuously stretching a neurite bundle in between. Using the
presented technique we achieved networks with wiring regions which are made
exclusively of neuronal processes unbound to the surface. The resulted networks
patterns are very stable and can be maintained for as long as 11 weeks. The
approach can be used to build advanced Neuro-chips
for bio-sensing applications (e.g drug and toxin
detection) where the structure, stability and reproducibility of the networks
are of great relevance.
Figure
1: Engineered neuronal network on CNT electrodes
Using
these engineered systems we also explored the properties of isolated clusters. Interestingly,
isolated neuronal clusters in vitro demonstrate clear oscillatory behavior. Using
our engineered systems, It is also possible to explore
how connected clusters develop hierarchical connectivity.
Figure
2: Oscillation in small neuronal clusters.
More:
·
http://www.newscientisttech.com/channel/tech/dn9391.html
·
http://www.sciencedaily.com/releases/2011/07/110712094203.htm
Compact self-wiring
in cultured neural networks
Journal of Neural
Engineering, 3 (2006) 95-101, Full text: pdf
Collaborators
Prof. Eshel Ben-Jacob (Physics, TAU Israel)
Dr. Danny Baranes, Pablo Blinder (BGU Israel)