Journal of Microwave Power and Electromagnetic Energy (JMPEE)

 

TITLE

Effect of 24 50 MHz Microwave Radiation on Horseradish Peroxidase [PDF]

AUTHORS

H.M. Henderson, K. Hergenroeder, and S. S. Stuchly

1975

10

1

27-36

YEAR

VOLUME

ISSUE

PAGES

 

Abstract

Solutions of horseradish peroxidase were subjected to 2450 MHz CW micro- wave radiation over an absorbed power density range of 62.5 to 375 W/cm³ for time periods of 10, 20, 30 and 40 minutes.  The enzyme samples were placed in a specifically designed waveguide applicator, and strict temperature control (25°C -± 1 to 2°C) was maintained by circulation of the coolant carbon tetrachloride. Our findings indicate that, in our experimental conditions, there is a significant inactivation of the enzyme only for absorbed power densities greater than 125 W/cm³ for 20 minutes, or at power densities above 60 W/cm3 for periods greater than 20 minutes. It is concluded that the dosages of microwave radiation required to achieve significant inactivation of the enzyme at 25°C are very high, and therefore any practical utilization of microwave power for inactivation of peroxidase at room temperature appears to be doubtful at the present time.