Wide-Field IPM Combined with Fluorescence

  • Based on Mach-Zehnder interferometer.
  • Supercontinuum low-coherence source in the input of the IPM system.
  • System is combined with fluorescence microscopy.
  • System is combined with a compact incubator for long experiments.
  •  
       

Applications:

  • Developing new IPM parameters that:
    • Do not require decoupling refractive index and physical thickness as a prior stage.
    • Are based on a combination of fluorescence and IPM.
  • Cancer cells:
    • Measuring membrane fluctuations as an indication for cancer.
    • Prediction of cell lifecycle stages using new IPM-based parameters.
  • Red blood cells (RBCs): Measuring membrane fluctuation maps.

P. Girshovitz and N. T. Shaked, “Generalized cell morphological parameters based on interferometric phase microscopy and their application to cell life cycle characterization,” Biomedical Optics Express, Vol. 3, No. 8, pp. 1757-1773, 2012 [PDF, Media 1] [Link].